Gene Cloning, Expression & Protein purification Services - Exporter & Manufacturer, Vadodara, India

Avail our expertise and facility to express your gene and get purified protein for further studies including raising of polyclonal or monoclonal antibody, assay development, etc. Starting from full-length cDNA clone, the gene would be cloned into an expression vector, sequence confirmed and the protein expressed would be purified. The services offered would include:

Subcloning of the full-length cDNA into an expression vector
Sequence confirmation of the gene by bi-directional sequencing
Optimization of protein expression
Purification (5-10 mg) of expressed protein, up to 90% purity

Features:

Sequencing of the gene after subcloning into expression vector would be undertaken if PCR based cloning techniques are employed.
Protein expressed may or may not be biologically active
Only E. Coli based expression systems available
GST and (His)₆ tags available for easy purification of the recombinant protein
Level of expression of recombinant protein may vary (very high to no expression at all) depending on toxicity of the protein to the E. Coli host
Facility for >90% purity available, please inquire
Subsequent raising of monoclonal or polyclonal antibody from the purified proteins, available (Catalogue nos. HS001 and SER 11 respectively)

Deliverables:

All clones and subclones generated
Expressed and purified recombinant Protein
Sequence data (raw data as well as analyzed and aligned data)
Final report containing relevant documents for the project

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