Introduction:
Characterization of genes would often require different cloning steps including making deletions in the up-stream regions for gene expression regulation studies, development of chimeras for modified proteins/enzymes, development of clones for transgenics, etc. Following are the different types of Gene Engineering services offered:
- Development of Deletion mutants for characterization of regulatory elements
- Development of Chimeras by cloning regulatory sequences up-stream or down-stream to the gene of interest
- Development of Knock-out constructs
- Development of constructs with multiple repeat elements
- Construction of recombinant clones starting from a given modified intermediate clone
Features:
- Sequence confirmed clones would be delivered
- Once the scientist designs the construct, we would formulate the cloning strategies and execute it
- Source for all DNA fragments to be cloned essentially comes from the scientist
- Recombinant clones with repeat sequences might become unstable
Deliverables:
- Sequence confirmed final clone DNA
- All intermediate clones developed
- All primers synthesized and left over at project completion
- Final Report
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