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SCAR primers for sample specific amplifications are developed and validated typically following the steps:
- Polymorphic RAPD profiles generated
- Polymorphic DNA fragments are cloned and sequenced
- SCAR primer sets are designed and synthesized
- SCAR primer validation by PCR
Features:
- Validated protocols
- Reproducible results
Deliverables:
- All clones developed and sequence data
- Primers synthesized and left over at project completion
- Final report with validated primers and protocols
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